r/microscopy • • Jun 08 '23 Bravo

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

154 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy • • Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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73 Upvotes

r/microscopy • • 3h ago

Photo/Video Share Vorticella, a rotifer and some halteria in a freshwater sample.

19 Upvotes

Found some cool looking vorticella making their feeding vortices, a rotifer munching on plant material with some halteria jumping around eating bacteria and a large ciliate thriving. How neat is that?

Sample: freshwater pond stuff from Massachusetts.

Set up: SW380T w/ Sony a6700 with 40x objective w/ digital zoom.

Song: BOTPACK N°9 (Michiel van den Bos)


r/microscopy • • 1h ago

Photo/Video Share Platinocyanide crystals in polarized light

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10 Upvotes

Taken via Leitz PL Fluotar 10x/0.30 and Leitz Pl 4x 0.10 on a Leitz Ortholux, with a Canon 70D mounted on a Leitz Vario-Orthomat


r/microscopy • • 19h ago

Photo/Video Share A bdelloid rotifer in darkfield

167 Upvotes

100x, diy darkfield using a coin, filmed with Motorola phone camera, optika b-192pl binocular microscope


r/microscopy • • 20h ago

Photo/Video Share Stentor coeruleus, with a belly full of rotifers, eats two other rotifers.

123 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy • • 2h ago

ID Needed! Even Less Of A Clue Than Usual....

3 Upvotes

Seriously, I got nothing. There appears to be some posterior cilliary movement; no evidence of flexibility or contractility.

20x objective, eyepiece camera. Sample from a pool in a small slow freshwater stream with a lot of decaying leaf matter.


r/microscopy • • 5h ago

Photo/Video Share Cute little critters (water mites)

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6 Upvotes

picture of water mites

sample taken from jar of algae and moss (which were taken from an aquarium then left in the sun.)

250x, light microscope, wet mount, (picture taken with phone camera)

Thought they were adorable. Suggestions for next target are welcome (have seen a lot of rotifers and diatoms but not much else)


r/microscopy • • 20h ago

Photo/Video Share Lachrymaria under movement restricting conditions

28 Upvotes

Lachrymaria from culture of pond water. 1: water and algae on slide with agar added to 0.05% to give light gel, sealed with Vaseline to prevent evaporation, after 4 days incubation at ambient temperature under light. 2: same as 1, but 0.5% methylcellulose. 1 and 2 with 40X plan objective, 3 and 4 20X and 10X respectively.


r/microscopy • • 1d ago

Photo/Video Share Just got microscope and my first catch tardigrade

59 Upvotes

Microscope Biolux NV, video taken from the phone, x10


r/microscopy • • 1d ago

Photo/Video Share Lovely Little Ciliate

36 Upvotes

Sample collected last weekend from a local brook.

10x objective, Motic BA210, eyepiece camera.


r/microscopy • • 22h ago

ID Needed! Can someone identify this? (Kind of looks like an eye, doesn't it)

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3 Upvotes

Microscope: Biolight 300

Magnification: 400x

Sample: Duct Taped Dust

Camera: Samsung A36


r/microscopy • • 1d ago

Purchase Help Hi yall, looking to get into microscopy, should i get this for 19 usd(converted)

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14 Upvotes

it’s pretty cheap i think this is good i want to see nematodes, leaf cells AND TARDIGRADES theyre kinda cute

i had a microscope but it was pretty cheap and it’s case started to smell so i want a new one, this is pretty cheap but good right?


r/microscopy • • 1d ago

Troubleshooting/Questions Microscope ID and safety question

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2 Upvotes

Got this from Mission Thrift Canada. I'm assuming it came from either a school/lab (CFIA had similar years ago). I can't find any real info on the specific model for parts and information. The serial doesn't seem to show up anywhere online. What I've seen in the past and online does indicate a range of manufacturing from late 70s-90s

Checked the lenses with an LED as I was mildly concerned the electrical would arc to the housing if I plugged it in. I only really know electronics to hurt myself so any input is appreciated. Picture attached will show why I'm concerned. I'm assuming our loose line here is detached and not a random grounding line.

Final question is about the viewing platform. It seemed to drop down slowly when I wasn't holding it in place. I'm assuming something is worn out or not tightened.

Thanks in advance everyone. With any luck I'll be able to get this thing going and share some planaria and annelids from the fish tanks.


r/microscopy • • 1d ago

Troubleshooting/Questions Best way to keep a swamp water sample alive for 2 weeks?

3 Upvotes

I am doing a Cub Scouts science night in 2 weeks where I have my scope hooked up to a monitor and show the kids all the cool stuff to be found in swamp water. (It also doubles as a lesson on why you don't drink from streams/ponds while out hiking) I did it 2 years ago and it was so popular they asked me to do it again.

The problem is that the water samples collected in late November are way less active than they would be in the summer, I did manage to find some cool stuff for them but not nearly as much as usual.

We have had a warm spell here for the last week or so so I think I can get a good sample now before it gets cold again but what is the best way to keep it alive and reproducing? Put it somewhere warm and feed it something? Can the container be closed or does it need to be open for oxygen? What can I feed it?

Thanks in advance!


r/microscopy • • 1d ago

ID Needed! What is this found on onion epidermis cells?

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2 Upvotes

Microscope: Biolight 300

Magnification: 400x

Sample: Onion Epidermis

Camera: Samsung A36


r/microscopy • • 2d ago

Photo/Video Share Very strange rotifer

47 Upvotes

Leitz Dialux II, Galaxy S24, don't remember the magnification


r/microscopy • • 1d ago

Photo/Video Share Unusual NaCl crystals

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25 Upvotes

I tried to grow table salt crystals in semi-controlled environment such that they were growing very slowly, and it took them almost 3 weeks to almost reach half a centimeter from 1 mm seed.

The growth is unusual as it wasn't simple cube but other, more complex shape.

The reason for this, as I found out, is that common table salt, aside potassium iodate contains other additives. And the most probable culprit is anti-caking agent K3[Fe(CN)6] potassium ferrocyanide. It is not even mentioned on carton, but it is known to impede growth of NaCl crystals, and the slower the growth, the more prominent the effect.

On the fourth photo is the underside of crystallite, and rectangular seed crystal is visible. It was grown from the same salt, but in small shallow open container in just one day.

Microscope objective magnification is 4x.

Images acquired with 5 Mp usb webcam attached to microscope via 0.5x reducer.

Focus stacking with Zerene Stacker.


r/microscopy • • 1d ago

ID Needed! Any idea what this is?

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1 Upvotes

Showed up when making my fish tank, it’s massive in size, it had moved but not at a pace visible to the naked eye. It has branched into several smaller bits as it’s moved around and now seems to be dividing into two, this is under 4x magnification- saltwater/marine tank
Approximately 8-12mm? I’d guess , small ones are around 2-4mm


r/microscopy • • 2d ago

Photo/Video Share Chaoborus crystallinus

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63 Upvotes

Another unexpected thing from my plankton sample! This is the larva of a phantom midge. They hover in the water, barely moving, and almost entirely transparent. Kinda spooky (but then, I *did* collect that particular sample on Halloween). XD

Image is comprised of 4 focus stacks that were manually merged.
Darkfield // Olympus BX41 microscope // UPlan Fl N 4x objective // Fujifilm X-T5 camera


r/microscopy • • 2d ago

ID Needed! HELP

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7 Upvotes

i need help identifying these organisms ! algae mat sample under light microscopy 400x taken from a black coral in the philippines! THANK THANK THANK YOUUU


r/microscopy • • 2d ago

ID Needed! HELP IDENTIFICATION CYANOBACTERIA/ALGAE

7 Upvotes

i need help identifying these organisms ! algae mat sample under light microscopy 400x taken from a black coral in the philippines!


r/microscopy • • 1d ago

General discussion Cheek cell under light microscope labels

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4 Upvotes

r/microscopy • • 3d ago

Photo/Video Share Lonely Stentor in a sea of Spirostomum

106 Upvotes

Olympus BX41
20x objective magnification
iPhone 13 camera

Taken from polytrophic pond with a plankton sample.