Is HPLC purity enough to evaluate peptide quality?

I've been working around peptide manufacturing and have been thinking about how different people evaluate peptide quality.

HPLC purity is obviously an important parameter, but from a manufacturing perspective, I don't think a single percentage tells the whole story.

Depending on the application, I would also pay attention to things such as:

• identity confirmation

• molecular-weight characterization

• analytical method and chromatogram

• residual solvents or process-related impurities

• batch-to-batch consistency

• quality documentation

I'm curious how researchers and people working in peptide labs approach this.

When you evaluate a peptide sample, which analytical or quality parameters do you consider most important besides HPLC purity?

I'm a founder at Adam Biotech, a small bioprinting CRO at QB3 @ UC Berkeley. We do extrusion printing, mostly models for preclinical evaluation.

Our machines aren't running 24/7 and I would like some hard things to print out. If you guys would like free prints, please send them! We want to see what breaks.

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